DNA polymerase stalling, sister chromatid recombination and the BRCA genes

A new role for Rrm3 in repair of replication-born DNA breakage by sister chromatid recombination

Replication forks stall at different DNA obstacles such as those originated by transcription. Fork stalling can lead to DNA double-strand breaks (DSBs) that will be preferentially repaired by homologous recombination when the sister chromatid is available. The Rrm3 helicase is a replisome component that promotes replication upon fork stalling, accumulates at highly transcribed regions and preve...

Mouse satellite DNA, centromere structure, and sister chromatid pairing

The experiments described were directed toward understanding relationships between mouse satellite DNA, sister chromatid pairing, and centromere function. Electron microscopy of a large mouse L929 marker chromosome shows that each of its multiple constrictions is coincident with a site of sister chromatid contact and the presence of mouse satellite DNA. However, only one of these sites, the cen...

UNEQUAL SISTER CHROMATID EXCHANGE ACTS ON Ig GENES

Though the phenomenon of sister chromatid exchange (SCE) 1 has now been known for over thirty years (1-3), its biological role(s) remains poorly defined (see ref. 4). The greatest number of observations of SCE have come from cytogenetic studies using agents such as [3H]thymidine or 5-bromodeoxyuridine to label sister chromatids differentially and observe their exchange. Such studies demonstrate...

Sister Chromatid Cohesion and Aneuploidy

Multiple recombination pathways for sister chromatid exchange in Saccharomyces cerevisiae: role of RAD1 and the RAD52 epistasis group genes.

Sister chromatid exchange (SCE) can occur by several recombination mechanisms, including those directly initiated by double-strand breaks (DSBs), such as gap repair and break-induced replication (BIR), and those initiated when DNA polymerases stall, such as template switching. To elucidate SCE recombination mechanisms, we determined whether spontaneous and DNA damage-associated SCE requires spe...